Assessing the Potential of NGF-Differentiated PC12 Cells as a Model for Synaptic Transmission.

ABSTRACT: PC12 cells, derived from rat pheochromocytoma, are widely used to model neuronal differentiation and disease. Upon nerve growth factor (NGF) stimulation, they adopt a neuron-like phenotype, offering insights into neuronal processes. However, their fidelity in mimicking neuronal presynaptic organization has been debated, and their lack of postsynaptic organization is apparent. This study evaluates NGF-differentiated PC12 cells for up to 21 days post-induction, focusing on synaptic vesicle-like vesicle (SVLV) recycling. Using immunofluorescence, correlative light and electron microscopy (CLEM), cryo-electron tomography, and electrophysiology, we assessed presynaptic-like architecture and functionality. By combining ultrastructural and functional modalities, we provide a more integrated evalu

SUBMITTER: Ott G 

PROVIDER: S-EPMC12696136 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature