Enhancing bovicin HC5 production in Streptococcus equinus HC5 through adaptive laboratory evolution under thermal stress.

ABSTRACT: Bovicin HC5, a bacteriocin produced by Streptococcus equinus HC5, demonstrates inhibitory activity against pathogenic and spoilage microorganisms. However, low production yields hinder its widespread application. This study investigated the impact of temperature on S. equinus HC5 growth and employed adaptive laboratory evolution (ALE) under heat stress to obtain variants with improved bovicin HC5 production. The optimal growth temperature for the wild-type strain was determined to be 42 °C, with growth ceasing above 49 °C. Following 400 generations of ALE at 47 °C and 48 °C, eight variants were selected. Two of these variants exhibited significantly enhanced bovicin HC5 production, reaching up to a 140% increase (P < 0.05). The variant with the highest bacteriocin yield showed increased ex

SUBMITTER: Dias RG 

PROVIDER: S-EPMC12696137 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature