Prevalence and characterization of carbapenem-resistant gram-negative bacteria from poultry in Tamil Nadu, India.

ABSTRACT: Recent emergence of carbapenem-resistant bacteria in poultry has raised concerns on the potential transmission to humans. We aimed to investigate the molecular epidemiology, genotype and phenotype of the carbapenem-resistant gram-negative bacteria isolated from raw chicken meat samples from India and to evaluate resistance and virulence genes through whole genome sequencing (WGS). Poultry samples (n = 250) from local vendors in Coimbatore, Tamil Nadu, India were enriched in selective McConkey broth before culturing on McConkey agar (both containing 4 μg/ml meropenem). The isolates were screened for carbapenem resistance genes and carbapenemase production by PCR and Modified Hodge test respectively. Selected isolates underwent WGS for detailed analysis of the bacterial genomes. Carba

SUBMITTER: Sivasankar S 

PROVIDER: S-EPMC12696407 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature