Engineered Decellularized Matrix Hydrogels with Crypt-Villus Topography for Forming Functional Intestinal Epithelium.

ABSTRACT: Creating a functional intestine model remains challenging owing to the complex topography of the intestinal crypt/villus structure. Here, an inverse molding biofabrication technique is presented to address this challenge. A sacrificial hydrogel mold (SHM) with negative crypt/villus features is first fabricated using digital light processing (DLP) 3D printing of poly(ethylene glycol)-norbornene-tyramine (PEGNB-T) thiol-norbornene hydrogels. Next, decellularized small intestine submucosa-norbornene (dSIS-NB) solution is cast and photopolymerized over the SHM, also via efficient thiol-norbornene photoclick reaction using PEG-tetrathiol as the crosslinker. The hydrogel construct is placed in a buffer solution to induce autonomous and rapid dissolution of the SHM, creating dSIS-NB hydrogels wit

SUBMITTER: Luong NH 

PROVIDER: S-EPMC12696786 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature