Unraveling the Folding Dynamics of DNA Origami Structures.

ABSTRACT: Achieving high folding yield remains a challenge in DNA origami, particularly as structures increase in complexity and scale. Here, how DNA origami design influences folding is investigated using a combination of real-time fluorometry, gel electrophoresis, electron microscopy, and theoretical analysis. Results reveal a balance of free energy changes from loop formation and hybridization that govern nucleation of nanostructure assembly, while the extent of cooperativity determines the overall assembly. The effect of structural complexity, staple design, and scaffold design on each energetic parameter, folding yield, kinetics, and cooperativity is measured. The results show that the scaffold pattern determines the extent of cooperativity, where fewer scaffold crossovers result in more cooper

SUBMITTER: Mohammadi-Zerankeshi M 

PROVIDER: S-EPMC12696799 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature