RIPK3 Protects Against Endothelial Activation and Vascular Permeability in a Mouse Model of Ischemia-Reperfusion Injury.

ABSTRACT:

Background

RIPK3 (receptor-interacting protein kinase 3) has context-specific roles that are frequently associated with cellular damage and death. We previously found that hypoxia can trigger elevated levels of RIPK3 in endothelial cells (ECs), which contributes to lethal vascular rupture during mouse embryonic development. However, it is unknown whether elevated RIPK3 likewise compromises endothelial barrier function in adult vasculature under hypoxic conditions such as ischemia-reperfusion (I/R) injury.

Methods

Twelve-week-old male and female littermate control or inducible EC-specific Ripk3 knockout (Ripk3^iECKO) mice were exposed to surgical intestinal I/R injury. Clodronate liposomes were used to reduce circulating monocytes in vivo. Immor

SUBMITTER: Johnson CF 

PROVIDER: S-EPMC12696878 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature