Genetic and epigenetic markers in the METTL21C gene associated with umbilical hernia in pigs.

ABSTRACT:

Background

Hernias, particularly umbilical hernias (UH), are prevalent anatomical anomalies in swine, leading to significant welfare issues and economic losses. Besides environmental factors also genetic components contribute to the development of UHs, though the exact mechanisms remain unclear. This study employed a multiple approaches integrating RNA-seq, DNA methylation analysis, Sanger sequencing, droplet digital PCR and western blot analysis to investigate the genetic and epigenetic underpinnings of UH in pigs. Muscle tissue from affected and control pigs was examined to identify differentially expressed genes (DEGs) and associated pathways.

Results

We found 59 significant DEGs, including SIM1, PITX1, HOXA7, METTL21C, PVALB, ALX1, EYA2, and TBX1. Interestingly, RNA-seq

SUBMITTER: Wozniak J 

PROVIDER: S-EPMC12696900 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature