FBXW7 regulates MAP7 through ubiquitination to affect the phenotypic characteristics and paclitaxel sensitivity of paclitaxel-resistant lung adenocarcinoma cells.

ABSTRACT:

Background

The emergence of resistance to paclitaxel (PTX) poses a major challenge to the effective treatment of lung adenocarcinoma (LUAD). This study aimed to elucidate the role of microtubule-associated protein 7 (MAP7) in regulating cell phenotypes and PTX sensitivity of PTX-resistant LUAD cells.

Methods

Two PTX-resistant LUAD cell lines (A549/PTX and HCC-827/PTX) were generated. The effects on cell apoptosis, migration, invasiveness, tube formation, colony formation, and proliferation were evaluated. Subcutaneous xenograft studies were conducted to assess the in vivo role. The regulatory mechanism of F-Box and WD repeat domain containing 7 (FBXW7) on MAP7 protein was examined through stability analysis and immunoprecipitation (IP) assay.

Results

In PTX-resistant

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12696929 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature