High-throughput cytokine detection platform for evaluation of chemical induced microglial activation.

ABSTRACT: Environmental chemical exposure, such as pesticides and heavy metals, may contribute to neurodegenerative disorders through neuroinflammation. This study aims to identify suitable in vitro microglial models for assessing cytokine responses to potential neurotoxicants, particularly focusing on human induced pluripotent stem cell-derived microglia (hiMG). In this study, we evaluated the cytokine secretion profiles of four microglial cell types-hiMG, HMC3, IM-HM, and BV2-upon stimulation with lipopolysaccharides (LPS) using cytokine arrays. Our findings showed cytokine response patterns in hiMG cells that most closely resemble in vivo conditions, with significant increases in interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) levels, the latter being uniquely expressed after LPS tre

SUBMITTER: Yang S 

PROVIDER: S-EPMC12697230 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature