Dynamic changes in mRNA isoform usage during human retinal development.

ABSTRACT:

Background

Alternative mRNA splicing is a key mechanism for generating isoform diversity in eukaryotic cells. However, the extent of the splicing changes that occur during complex regulatory processes like neurodevelopment are still incompletely characterized.

Results

We performed nanopore-based long-read RNA sequencing on differentiating human stem cell-derived retinal organoids to identify temporal patterns of isoform usage across developmental stages. We found that retinal organoids undergo dynamic shifts in isoform usage throughout differentiation, which were not necessarily accompanied with changes in overall gene expression, as was the case for many genes involved in the regulation of mRNA splicing itself. Further analysis of human stem cell-derived retinal ganglion ce

SUBMITTER: Keuthan CJ 

PROVIDER: S-EPMC12697546 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature