U1 RNA Detected by Toll-Like Receptor 3 Plays a Role in the Pathogenesis of Pterygium.

ABSTRACT:

Purpose

Toll-like receptor 3 (TLR3) detects RNA from pterygium epithelial cells (PECs). We previously suggested that pterygium development may be linked to RNA released from abnormally growing PECs: U1 RNA released from ultraviolet B (UVB)-damaged cells may activate TLR3. This study investigated how U1 RNA, polyriboinosinic:polyribocytidylic acid (poly[I:C]), and PEC lysates affect the TLR3 signaling pathway in PECs and conjunctival epithelial cells (CECs).

Methods

Human pterygium and ipsilateral pterygium-free conjunctiva from the same patients were used for cell culture and RNA-sequencing analysis. PECs and CECs were cultured, irradiated with UVB, and treated with poly(I:C), PEC lysates, or synthetic U1 RNA. TLR3 and toll/interleukin-1 receptor domain-containing adaptor-in

SUBMITTER: Lai CC 

PROVIDER: S-EPMC12697701 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature