Single-Cell Nucleus Extraction with Cellular Indexing.

ABSTRACT: Bulk organelle‑fractionation masks cell‑to‑cell heterogeneity, and existing microfluidic methods cannot reliably reconnect each isolated organelle to its parent cell, an essential capability for multiomics readouts. VacTrap, a high-throughput microfluidic device that isolates and spatially indexes single nuclei from mammalian cells is developed. The VacTrap device consisted of three aligned layers: 1) a Bis-gel microwells layer with a "trapdoor" (BAC-gel) base, fabricated atop a through-hole glass slide; 2) a polydimethylsiloxane (PDMS) microwell layer to receive transferred nuclei; and 3) a vacuum manifold. VacTrap operation begins with cell cytoplasmic lysis using differential detergent fractionation (DDF) to release intact nuclei into the Bis-gel microwells, while cytoplasmic proteins a

SUBMITTER: Lam T 

PROVIDER: S-EPMC12697769 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature