Generation of a primary culture of chick embryo enterocytes to evaluate the effects of fumonisin B1 and deoxynivalenol on cell morphology, actin filaments and nuclei.

ABSTRACT: Fumonisin B1 (FB1) and deoxynivalenol (DON) are among the most impactful mycotoxins affecting poultry production. The negative effects of these mycotoxins on intestinal epithelial morphology and integrity have been previously described. However, cell culture methods using cells from species with different genetic backgrounds have been used to obtain these results. The objective of this work was to evaluate the damage caused by fumonisin B1 (FB1) and deoxynivalenol (DON) on cell morphology, actin filaments and nuclear morphology in primary enterocytes obtained from chick embryos via fluorescence microscopy. The results revealed that FB1 and DON did not induce actin filament depolymerization, and stress fibers remained intact under the concentrations of mycotoxins evaluated. However, the cyt

SUBMITTER: Uribe-Rivera J 

PROVIDER: S-EPMC12697969 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature