Comparative evaluation of five rapid PCR platforms for respiratory virus detection.

ABSTRACT:

Background

The demand for rapid molecular diagnostics for respiratory viruses has increased substantially. Several point-of-care PCR platforms have become available, yet comparative performance data remain limited.

Objectives

To evaluate the diagnostic accuracy and operational reliability of four rapid PCR platforms for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A and B viruses (IAV, IBV), and respiratory syncytial virus (RSV), in comparison with the GeneXpert (Cepheid, USA) platform.

Methods

Nasopharyngeal swabs from patients with respiratory symptoms were tested using the GeneXpert, positive samples were subsequently analysed on four alternative systems: the 30-minute and 1-hour M10 (SD Biosensor, South Korea) assays, FlashD

SUBMITTER: Smit PW 

PROVIDER: S-EPMC12697972 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature