Combined nonmyeloablative total lymphoid irradiation and low-dose total body irradiation enhances donor chimerism and leads to islet allograft acceptance.

ABSTRACT: The nonmyeloablative conditioning regimen comprising total lymphoid irradiation (TLI) and antithymocyte globulin is used for allogeneic hematopoietic stem cell transplant patients. There is a need to safely intensify TLI+antithymocyte globulin to allow for the achievement of sustained mixed chimerism, particularly in cases where pre-existing autoimmunity poses a significant barrier to successful engraftment. We developed a nonmyeloablative immune preconditioning protocol using low-dose total body irradiation (TBI) to increase the level and durability of mixed donor chimerism. Non-obese diabetic mice were preconditioned with a modified protocol comprising TLI, low-dose TBI, and antithymocyte serum before bone marrow (BM) transplantation. The incorporation of low-dose TBI resulted in enhance

SUBMITTER: Pathak S 

PROVIDER: S-EPMC12698175 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature