Development of Multiplex PCR assay for detection of Canine Infectious Respiratory Disease Complex (CIRDC) pathogens in dogs.

ABSTRACT:

Background

Canine Infectious Respiratory Disease Complex (CIRDC) is a highly contagious, multifactorial syndrome that primarily affects dogs in crowded environments such as shelters, kennels, and breeding facilities. Three major CIRDC-associated pathogens: Canine distemper virus (CDV), Canine adenovirus type 2 (CAV-2), and Bordetella bronchiseptica (Bb) have been reported in the canine population of India.

Materials and methods

In this study, a multiplex PCR (mPCR) assay was developed and optimized for the simultaneous detection of these three pathogens. The multiplex assay was designed targeting three genes H, E3 and bfrZ of CDV, CAV-2 and Bb respectively. This multiplex assay was optimized in both singleplex and multiplex formats by adjusting key PCR paramete

SUBMITTER: Kaul R 

PROVIDER: S-EPMC12698605 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature