Electrostatically driven fluorescent sensor for rapid detection of AChE activity and organophosphate pesticides via dual-enzyme cascade amplification.

ABSTRACT:

Introduction

The widespread use of organophosphorus pesticides (OPs) in Chinese herbal medicines cultivation raises urgent concerns about residue contamination. Conventional detection methods [e.g., gas chromatography-mass spectrometry (GC-MS) and enzyme-linked immunosorbent assay (ELISA)] suffer from poor portability and instability of antibody inactivation in complex matrices, hindering on-site analysis.

Methods

This study proposed a novel "electrostatic adsorption-driven cascade reaction chain" strategy for rapid detection of acetylcholinesterase (AChE) activity and OPs. Leveraging the electrostatic self-assembly between a positively charged acetylcholine chloride (ACh, 26.47 ± 1.63 mV) and a negatively charged choline oxidase (CHO, -30.81 ± 1.85 mV), a nanoscale fluoresc

SUBMITTER: Liu X 

PROVIDER: S-EPMC12698611 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature