Periodontitis-associated metabolite isoleucine impairs intestinal barrier function and exacerbates intestinal inflammatory response by NF-κB signaling.

ABSTRACT:

Objective

Periodontitis-associated metabolite isoleucine (Ile) plays an important role in periodontitis aggravating colitis. However, how Ile exacerbates colitis is largely unknown.

Methods

C57BL/6J mice were used to establish experimental periodontitis and colitis models. Histological alterations of the periodontium and colon were observed by HE staining. The gut barrier function was evaluated by intestinal permeability using FITC-dextran. The expression of tight junctions (ZO-1 and occludin) was detected by immunohistochemical staining or immunofluorescence. The NF-κB signaling pathway was detected using qRT-PCR and Western blot.

Results

Experimental periodontitis and periodontitis-associated metabolite Ile increased the intestinal permeability, downregulated the ex

SUBMITTER: Wang X 

PROVIDER: S-EPMC12698614 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature