Diacetyl-induced bronchiolitis obliterans involves ubiquitin C upregulation and fibrosis-related gene activation in rats.

ABSTRACT:

Introduction

Bronchiolitis obliterans (BO) is an irreversible, chronic obstructive pulmonary disease with inflammation and fibrosis causing bronchiolar narrowing. Inhaling diacetyl (DA) can result in BO in humans.

Methods

We aimed to investigate the relationship between fibrosis-related gene expression and ubiquitin C (UbC) regulation in a rat model of BO following a single DA instillation, by examining lung histopathology, UbC protein levels, and transcriptomic changes.

Results

After DA exposure, rat bronchioles exhibited marked inflammation, increased collagen deposition, airway fibrosis, and obstruction. These changes were confirmed by histology and semi-quantitative image analysis. UbC protein levels were significantly elevated in a time-dependent manner. RNA sequ

SUBMITTER: Wang H 

PROVIDER: S-EPMC12698636 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature