A novel β-carboline alkaloid derivative targeting MDM2-p53 pathway suppresses colorectal cancer progression.

ABSTRACT:

Purpose

Colorectal cancer (CRC) remains a major global health challenge, necessitating novel therapeutic approaches. β-carboline alkaloids, natural compounds with anticancer properties, have shown potential to inhibit cancer cell viability. Here, we synthesized β-carboline derivatives and explored their potential as CRC inhibitors.

Methods

The IC₅₀ values of β-carboline derivatives were determined by cell viability assay. The biological effects of the leading candidate were evaluated via cell cycle analysis, proliferation assay, colony formation, apoptosis assay, and reactive oxygen species detection. Mechanistic studies were performed using transcriptomic and proteomic analysis, validated by immunoblotting, pulldown assay, cycloheximide-chasing assay, and co-immu

SUBMITTER: Zeng F 

PROVIDER: S-EPMC12698734 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature