Gut Phage Biobank: a collection of bacteriophages targeting human commensal bacteria.

ABSTRACT: The scarcity of cultivated gut bacteriophages hinders gut microbial research and application. Here we report the establishment and characterization of a Gut Phage Biobank (GPB) ( https://db.cngb.org/genomics/datasets/GDS0000055 ) through a systematic isolation workflow and containing 104 isolates that target abundant or disease-associated gut bacteria. Genomic analysis reveals high diversity among these phages, and key genes of phage-bacteria interactions. The infection matrix demonstrates high host-specificity and varying infectivity of these phages under different conditions, unveiling phage-bacteria interaction mechanisms. In-depth characterization of the phages targeting obligate anaerobes uncovers a previously undescribed family and four previously undescribed genera, one of which is

SUBMITTER: Xing B 

PROVIDER: S-EPMC12698836 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature