Layer 5 myelination gates corticothalamic coincidence detection.

ABSTRACT: Myelin is essential for the rapid conduction of action potentials (APs) but its role in long-range processing of disparate inputs remains unclear. Here, using a cell-type-specific approach we recorded optogenetically evoked pyramidal neuron responses via in vivo juxtacellular patch-clamp and Neuropixels probes, tracking spike transmission from layer 5 (L5) to the posteromedial thalamic nucleus (POm) in the mouse. Cuprizone-induced demyelination caused millisecond-scale delays, increased temporal jitter and impaired transmission of high-frequency AP bursts. Computational modeling of the saltatory propagation from L5 to POm revealed that myelin loss from neocortical internodes acts as a low-pass filter, impeding high-frequency spikes within the burst. Finally, pairing optogenetic stimulation

SUBMITTER: Jamann N 

PROVIDER: S-EPMC12699038 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature