Investigation Into the Role of Reductants and Cosubstrates in Lytic Polysaccharide Monooxygenase Thermothielavioides terrestris AA9E Binding to Cellulose by Single-Molecule Imaging.

ABSTRACT: Cellulose-active Lytic Polysaccharide Monooxygenases (LPMO) facilitate plant cell wall deconstruction by attacking ordered regions of cellulose. In vitro, reductants (e.g., ascorbic acid) reduce LPMOs to Cu(I)-LPMO, and hydrogen peroxide (H₂O₂) serves as co-substrate for oxidative cleavage of cellulose glycosidic bonds. Super-resolution single-molecule imaging by total internal reflection fluorescence microscopy was used to visualize and enumerate binding events of fluorescently-labeled Thermothielavioides terrestris AA9E (TtAA9E) on highly ordered cellulose fibrils in oxygen-scavenging buffer systems. In the glucose oxidase/catalase (GODCAT) system, oxygen is converted to H₂O₂, then removed by catalase. Adding ascorbic acid to the GODCAT system

SUBMITTER: Blossom BM 

PROVIDER: S-EPMC12699106 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature