Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.

ABSTRACT: Chinese hamster ovary (CHO) cells, widely utilised in biopharmaceutical production, experience various stressors during cell culture that can affect protein expression and folding, particularly within the endoplasmic reticulum (ER). Mild hypothermia is widely employed in CHO cell bioproduction to improve recombinant protein yield and quality; however, its impact on ER-associated pathways, particularly those governing protein folding and stress responses, remains insufficiently characterised. Mass spectrometry-based proteomics allows for the identification and relative quantification of proteins, enabling detailed insights into protein expression, modifications, and functional networks. This study investigates the impact of mild hypothermic conditions (31°C) on the whole cell proteome and u

SUBMITTER: Ryan D 

PROVIDER: S-EPMC12699125 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature