Comprehensive Mapping of Functional Enhancers in Chinese Hamster Ovary Cells.

ABSTRACT: Chinese hamster ovary (CHO) cells are the leading mammalian system for recombinant therapeutic protein production. However, optimizing transgene expression remains challenging due to the limited understanding of the regulatory mechanisms controlling gene expression in CHO cells. Towards overcoming this barrier, here we provide a systematic characterization of cis-regulatory elements in CHO cells. Using genome-wide STARR-seq, a high-throughput method for quantifying enhancer strength, we identified regions with enhancer activity in the CHO cell genome. By integrating these data with ATAC-seq and histone modification profiles, we were able to characterize the chromatin state of these regions. Our analysis revealed thousands of newly identified enhancer sequences. The most active sequences co

SUBMITTER: Santos M 

PROVIDER: S-EPMC12699134 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature