Quantitative Analysis of DNA Double-Strand Breaks in Genomic DNA Using Standard Curve Method.

ABSTRACT:

Background

DNA double-strand breaks (DSBs) are the most lethal and dangerous type of lesions with significant implications for both cellular function and organismal health. The number of DSBs (N_DSBs) across the genome reflects DNA damage severity. However, current quantification methods mainly rely on next-generation sequencing, which is laborious and expensive. This study aims to provide a simple, low-cost, and high-throughput standard curve-based method for quantifying genome-wide DSBs.

Method

Genomic DNA from human, mouse, Arabidopsis, Saccharomyces cerevisiae, and Escherichia coli was digested by seven blunt-end restriction enzymes to generate DSB standards. Theoretical N_DSBs for each standard were calculated based on restriction site frequency. Lig

SUBMITTER: Guo L 

PROVIDER: S-EPMC12699195 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature