Mycobacteriophage Yasnaya_Polyana and its engineered lytic derivative: specificity of regulatory motifs and lytic potential.

ABSTRACT:

Introduction

The growing prevalence of multidrug-resistant Mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) highlights the urgent need for alternative therapeutic approaches. Mycobacteriophages, viruses that selectively infect mycobacteria, have emerged as promising tools. Here, we report the isolation and characterization of a new subcluster K4 phage, Yasnaya_Polyana, with a focus on its regulatory motifs and engineered lytic variant.

Methods

The phage was isolated by enrichment on Mycobacterium smegmatis mc(2)155, followed by genome sequencing and functional annotation. Start-Associated Sequences (SAS) and Extended SAS (ESAS) were analyzed in silico across 188 cluster K phages. A lytic derivative, YPΔ47, was engineered by deleting the r

SUBMITTER: Shitikov E 

PROVIDER: S-EPMC12699233 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature