Impact of changed c-di-AMP levels and hypoosmotic stress on the transcriptome of <i>Haloferax volcanii</i> and on RCK domain-containing proteins.

ABSTRACT: We investigated the role of cyclic di-adenosine monophosphate (c-di-AMP) in the halophilic archaeon Haloferax volcanii by analysing transcriptomic changes in a strain with lowered c-di-AMP levels and by characterizing the function of key RCK (regulator-of-conductance-of-K⁺) domain proteins. The c-di-AMP-reduced mutant showed elevated expression of cell division genes and metabolic enzymes, whereas a Na⁺/H⁺ antiporter and an aspartate aminotransferase were strongly repressed. These patterns reveal previously unknown links between this messenger and both cell division and osmolyte homeostasis. To probe downstream effectors, we created deletion mutants of four RCK domain proteins and observed distinct phenotypes under potassium or sodium limitation. Deleting the primary RCK protein, li

SUBMITTER: Ren H 

PROVIDER: S-EPMC12699388 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature