Identification and validation of microglia-associated genes in ischemic stroke using single-cell and bulk RNA-seq.

ABSTRACT: Ischemic stroke (IS) is an acute cerebrovascular disease characterized by high incidence and mortality. The mechanism of microglia in the pathogenesis of IS remains unclear. This study aimed to explore the key genes related to microglia in IS and their molecular mechanisms in the pathogenesis. In this study, the transcriptome data of IS were retrieved from public databases. Subsequently, candidate genes were identified through the intersection of microglia-related genes (MGGs) obtained via single-cell annotation and high-dimensional weighted gene co-expression network analysis (hdWGCNA) with differentially expressed genes (DEGs). Next, key genes were determined through protein-protein interaction (PPI) analysis and verification of expression levels. Afterwards, enrichment analysis, variati

SUBMITTER: Qian D 

PROVIDER: S-EPMC12699842 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature