A single-cell atlas of RNA alternative splicing in the glioma-immune ecosystem.

ABSTRACT:

Background

Single-cell analysis has refined our understanding of cellular heterogeneity in glioma, yet RNA alternative splicing, a critical layer of transcriptome regulation, remains underexplored at single-cell resolution. Here, we present a pan-glioma single-cell alternative splicing analysis in both tumor and immune cells through integrating seven SMART-seq2 datasets of human gliomas to uncover overlooked isoform-level regulations shaping glioma progression and immune responses.

Results

Our analysis reveals lineage-specific alternative splicing regulation among glioma cellular states, with the most divergent alternative splicing landscapes observed between mesenchymal and neuronal-like glioma cells. Notably, this includes events in TCF12 exon 15 and PTBP2 exon 10, two key

SUBMITTER: Song X 

PROVIDER: S-EPMC12699894 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature