Rapid clinical detection of mycobacterial infections using M-Typer and flow-injection tandem mass spectrometry.

ABSTRACT:

Objectives

We evaluated the utility of the M-Typer® assay, based on flow-injection tandem mass spectrometry mycolic acid profiling, in the diagnosis of TB and other mycobacterial infections.

Methods

The ability of the M-Typer® assay to identify Mycobacterium species was first evaluated using 40 clinical strains of known species. In the second phase, clinical specimens from 451 patients with suspected TB or non-tuberculous mycobacteria (NTM) infections were tested. Results were compared with those obtained from Xpert MTB/RIF and MGIT culture.

Results

The M-Typer® assay correctly classified all clinical strains as either Mycobacterium tuberculosis complex or NTM, and 80% were accurately identified to species level. In clinical specimens, it showed high sen

SUBMITTER: Glogowska A 

PROVIDER: S-EPMC12699965 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature