Yeast-derived low-purity FGF2 supports bovine ESC and MSC aggregates in suspension.

ABSTRACT: Fibroblast Growth Factor 2 (FGF2) is an essential component of media for cultivated meat production, supporting the proliferation, survival, and differentiation of various cell types. The high cost of recombinant FGF2, typically produced in Escherichia coli (E. coli) with laborious downstream processing, remains a major bottleneck to commercial scalability. In this study, we developed a low-cost production platform using Pichia pastoris (P. pastoris), a food-grade yeast capable of secreting properly folded proteins into the culture medium. We produced bovine FGF2 and purified it using tangential flow filtration (TFF), omitting chromatography to reduce processing complexity and cost. The biological activity of these low-purity FGF2 variants was assessed in two relevant

SUBMITTER: Savyon G 

PROVIDER: S-EPMC12699982 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature