Identification of the body fluid donors from mixture stains using bulk transcriptomes data.

ABSTRACT: The deconvolution of the mixture contributors is essential but always presents challenges in crime scene investigation, especially when encountering mixtures with multiple contributors. In recent years, RNA-based genotyping has shown great advantages in assigning a body fluid to a specific mixture contributor, whereas transcriptome sequencing has been seldom applied for mixture deconvolution. In this study, we investigated the feasibility of bulk transcriptomes data for deconvoluting biological mixtures, and described a novel approach, utilizing bulk transcriptomes data of multi-body-fluid mixtures to identify body fluid donors. Computational deconvolution methods were introduced here to infer body fluid proportions from bulk transcriptomes data, and SNPs corresponding to a specific body f

SUBMITTER: Yu H 

PROVIDER: S-EPMC12700097 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature