Conformational Dynamics of PKM2 Regulate Hypoxia-Induced Pathological Retinal Angiogenesis.

ABSTRACT:

Purpose

Because anti-vascular endothelial growth factor therapies have limited effect in some cases of retinal neovascularization, there might be other potential pathways involved. Pyruvate kinase M2 (PKM2), a major isoform of pyruvate kinase in tumor cell and human retina, has been broadly studied in oncology and believed to play key roles in tumor growth and invasion. However, its role in retinal angiogenesis remains unclear. In this study, we aimed to explore the contribution of PKM2 conformational dynamics on pathological neovascularization of the retina.

Methods

We used the oxygen-induced retinopathy (OIR) mouse model and the hypoxia-exposed human retinal microvascular endothelial cell (HRMEC) model to evaluate PKM2 conformational dynamics. DASA-58, a small-molecule act

SUBMITTER: Zhu P 

PROVIDER: S-EPMC12700175 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature