DNA-directed electrochemiluminescence nanosphere with electrocatalysis-enhanced microfluidic arrays for rapid multibacterial detection.

ABSTRACT: Rapid detection of multibacterial pathogens is crucial for accelerating the diagnosis and treatment of bacterial infections. We propose a rapid and efficient electrochemiluminescence (ECL) sensor for the synchronous detection of multiple bacterial pathogens, including Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and methicillin-resistant S. aureus. This homogeneous sensor is based on a self-assembled DNA nanosphere loaded with tetrakis (4-carboxyphenyl) porphyrin (TCPP). The sensor operates in an "off-on" mode, in which bacterium-aptamer binding triggers a conformational change in the DNA nanosphere, releasing TCPP and generating an enhanced ECL signal. The inclusion of cerium nanoparticles boosts signal intensity through electrocatalyti

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12700205 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature