A fluorescent probe for concurrent detection of cysteine, homocysteine, and superoxide anion.

ABSTRACT: Redox imbalance is a key factor in the pathogenesis of diseases such as epilepsy and liver injury. Superoxide anion (O₂^•-), cysteine (Cys), and homocysteine (Hcy) play central roles in maintaining redox homeostasis, and their dysregulation drives oxidative stress and disease progression. Here, we report a multifunctional fluorescent probe, BPC, capable of simultaneously and selectively detecting Cys, Hcy, and O₂^•- in complex biological environments. BPC shows high sensitivity, selectivity, and biocompatibility, enabling real-time visualization of redox fluctuations in living cells and zebrafish with minimal cytotoxicity. In pentylenetetrazole (PTZ)- and acetaminophen (APAP)-induced models of epilepsy and liver injury, BPC revealed notable alterat

SUBMITTER: Li Y 

PROVIDER: S-EPMC12700212 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature