Droplet-based single-cell pairing for high-throughput interaction mapping of antigen-receptor combinations.

ABSTRACT: Mapping the interaction potential of different variant combinations of viral antigens and human cell receptors and understanding how viral antigen mutations interact with human genetic polymorphisms are critical for predicting infection susceptibility and informing precision public health strategies. Here, we develop a droplet-based single-cell pairing and library-on-library interaction screening (SPLIS) system for high-throughput profiling of the syncytium-formation landscapes of various spike-angiotensin-converting enzyme 2 (ACE2) variant combinations. This system uses combined droplet sorting and merging to deterministically encapsulate one antigen-presenting sender cell and one receptor-expressing receiver cell into each drop, followed by selection and sequencing of the fused DNA reado

SUBMITTER: Nan L 

PROVIDER: S-EPMC12700217 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature