Metabolomic profiling, antioxidant activity, and skin cell viability of citrus peel flavonoids extracted via ultrasonic-assisted aqueous two-phase system.

ABSTRACT: Citrus peels, often considered agricultural waste, are rich in flavonoids with potent antioxidant properties. This study utilized ultrasonic-assisted aqueous two-phase extraction (UA-ATPE) to obtain flavonoid-rich upper phase extracts (UPEs) from the peels of four citrus varieties sourced from different cultivars and regions. Targeted metabolomics was used to analyze the flavonoid compositions in the UPEs, revealing distinct metabolic profiles among the varieties. The antioxidant activities of the UPEs were evaluated through hydroxyl, superoxide, and ABTS radical scavenging assays, while their effects on skin cell viability were assessed using HaCaT and BJ cells. Multivariate statistical analyses, including principal component analysis (PCA) and hierarchical clustering analysis (HCA), iden

SUBMITTER: Lin S 

PROVIDER: S-EPMC12700371 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature