UTX/Top2β axis mediated spinal cord microvascular endothelial cells senescence exacerbates spinal cord injury.

ABSTRACT:

Background

Cellular senescence plays a critical role in regulating angiogenesis. While UTX has been implicated in vascular regeneration following spinal cord injury (SCI), its underlying mechanisms remain incompletely understood. This study investigates how senescence mediates UTX-regulated vascular responses after SCI.

Methods

We assessed p16INK4a (a senescence marker) and UTX expression in spinal cord endothelial cells at 3, 5, 7 and 14 days post-SCI. By breeding UTXflox/flox mice with heterozygous Tek-Cre mice, we generated endothelial-specific UTX knockout mice. Cellular senescence and proliferation were evaluated in primary spinal cord microvascular endothelial cells (SCMVECs). RNA sequencing and chromatin immunoprecipitation (ChIP)-seq were performed to identify UTX ta

SUBMITTER: Liu D 

PROVIDER: S-EPMC12700428 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature