Spatial Profiling Identifies Tumor-Associated Stroma Enrichment and MIF as Potential Immunotherapy Targets in Primary Ewing Sarcomas.

ABSTRACT:

Purpose

The tumor microenvironment (TME) plays a vital role in cancer survival and progression and may play roles in drug resistance and immune escape. To date, few studies have detailed the TME of Ewing sarcoma.

Experimental design

We performed spatially resolved transcriptomics of primary treatment-naïve Ewing sarcoma tumor biopsies from patients with or without clinical metastasis, complemented by high-plex spatial proteomic analysis.

Results

We discovered greater stromal enrichment in localized Ewing sarcoma primary tumors compared with metastasis-associated Ewing sarcoma primary tumors. Through spatial ligand-receptor analysis, we showed that the stroma-enriched regions harbor unique extracellular matrix-related cytokines, immune recruitment, and proinflammatory

SUBMITTER: Kuo C 

PROVIDER: S-EPMC12700766 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature