Nicotinamide mononucleotide treatment improves spermatogenesis in obese mice by reducing lysine acetylation of lactate dehydrogenase C.

ABSTRACT: Obesity is associated with impaired spermatogenesis and decreased sperm quality, in part through reducing Sertoli cells (SCs) lactate and nicotinamide adenine dinucleotide (NAD⁺) production. It is not known whether nicotinamide mononucleotide (NMN) treatment improves spermatogenesis. In the present study, NMN improved lipid metabolism and enhanced spermatogenesis in obese mice, alleviated SCs dysfunction in vivo and in vitro. Label-free quantitative acetylomics analysis of mouse testes suggested that protein acetylation influenced both the structural and functional properties of metabolic proteins. The beneficial effects of NMN were due in part to changes in the acetylation of glycolysis-related proteins. Furthermore, multi-omics and correlation analyses demonstrated that intera

SUBMITTER: Liu K 

PROVIDER: S-EPMC12700873 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature