Deletion or overexpression of tpx gene alters doubling time and response to stress agents and affects asphaltene biotransformation in Micrococcus sp. IITD107.

ABSTRACT: Thiol peroxidases have been identified as antioxidant enzymes in different organisms. However, there have been only a few reports on deleting or overexpressing its gene to characterize its function. Recently, the ability of the enzyme thiol peroxidase belonging to the Micrococcus sp. IITD107 on asphaltene biotransformation has been reported. Here, we report the construction of deletion and overexpression strains of tpx and compared them with the wild-type strain of Micrococcus sp. IITD107. For this purpose, whole-genome sequencing of the strain was carried out. The genome sequence revealed the presence of only one copy of the tpx gene and several other genes which might play a role in stress response, polyaromatic hydrocarbon (PAH) degradation, etc. The tpx deletion mutant was constructed

SUBMITTER: Patil N 

PROVIDER: S-EPMC12700925 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature