Cellulase secretion by engineered Pseudomonas putida enables growth on cellulose oligomers.

ABSTRACT: Pseudomonas putida is an attractive synthetic biology platform organism for chemical synthesis from low-grade feedstocks due to its high tolerance to chemical solvents and lignin-derived small molecules that are often inhibitory to other biotechnologically relevant microorganisms. However, there are few molecular tools available for engineering P. putida and other gram-negative bacteria to secrete non-native enzymes for extracellular feedstock depolymerisation. In this study, P. putida was transformed to secrete cellulase enzymes and evaluated for growth on polymeric or oligomeric cellulose substrates. Active exo- and endocellulase enzymes were secreted into the culture supernatant, and a preferred set of twin-arginine translocase secretion signal peptides were identified. Extracellular ce

SUBMITTER: Smith MR 

PROVIDER: S-EPMC12700990 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature