Systemic downregulation of EV-associated MiRNAs following remote ischemic preconditioning.

ABSTRACT: Remote ischemic preconditioning (RIPC) is a non-invasive strategy to protect tissues from ischemia-reperfusion injury (IRI), yet its systemic molecular mediators remain incompletely understood. Extracellular vesicles (EVs), particularly their microRNA (miRNA) cargo, have emerged as potential conveyors of RIPC-induced protection. In this translational study, we performed paired EV-miRNA profiling in five human subjects undergoing free flap surgery, with plasma collected before and 30 min after a standardized RIPC protocol. EVs were isolated, RNA was extracted and profiled using a 384-plex qPCR array. Differential expression was assessed using paired statistics and false discovery correction. Functional impact was inferred via validated target mapping, gene set enrichment analysis (GSEA), an

SUBMITTER: Drysch M 

PROVIDER: S-EPMC12701078 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature