Electrochemical Characterization of Site-Specifically Metal-Modified DNA Films on Gold Electrode Surfaces.

ABSTRACT: The electrochemical characterization of DNA films with different base mismatches or with Cu^II- or Ag^I-mediated pairs was carried out to assess possible immobilization and interaction effects. Toward this end, 3-hydroxy-2-methylpyridin-4(1H)-one (H), imidazole-4-carboxylate (K), purine-6-carboxylate (P), and 7-deaza-6-pyrazolylpurine (D) were used as artificial metal-binding nucleobases. Cyclic voltammetry and square-wave voltammetry confirmed the immobilization of suitably modified oligonucleotides on Au electrodes. The incorporation of the metal ions into the base mismatches to form metal-mediated base pairs showed a negligible effect on the peak potentials. Ambiguous electrochemical impedance spectroscopy results were obtained for DNA with metal-mediated base pairs,

SUBMITTER: Flothkotter N 

PROVIDER: S-EPMC12701301 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature