Ras Homolog Enriched in Brain Protein Reverses Amyloid Beta-Induced Escape of Inflammatory Cytokine mRNAs From Immunoisolated RNA Processing Bodies of Glioblastoma Cells.

ABSTRACT: Post-transcriptional regulation by RNA processing bodies, also known as P-bodies (PBs), is vital for mRNA translation, localization, and stability in various animal cells, including neurons and glial cells. PBs enable spatial control of protein synthesis, influencing differentiation and synaptic function. Understanding mRNA storage within PBs is essential for identifying the mechanism controlling post-transcriptional gene expression in brain cells and for understanding its role in neurodegenerative diseases. We developed a detergent-based method to isolate phase-separated P-bodies, free of cytosolic factors and RNAs, enabling us to analyze the mRNA content of PBs under different cellular or experimental conditions. Using neuronal (NGF-differentiated PC12) and glial cell (C6 glioblastoma) m

SUBMITTER: Ray S 

PROVIDER: S-EPMC12701684 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature