Immunohistochemical evaluation of T cell receptor and T cell receptor beta constant 1 expression distinguishes benign and neoplastic immature T-cell populations and reveals discrete TRBC1/TCR phenotypes.

ABSTRACT:

Background

Expression of TRBC1 and TRBC2 is increasingly assessed in the evaluation for clonal T-cell populations. While flow cytometry has repeatedly shown utility in evaluating TRBC 1/2 expression, immunohistochemical (IHC) methods have not been extensively examined, particularly in immature T-cell populations.

Purpose

This study evaluated TRBC1 IHC staining in formalin-fixed paraffin-embedded (FFPE) tissue, encompassing benign thymic tissue, thymomas, and T-lymphoblastic leukemias/lymphomas (T-LL).

Methods

IHC for TRBC1, CD3, TCR-BF1, and TCR-δ was performed in all cases; TdT was performed on all T-LLs. TRBC1 was scored as positively restricted (≥ 85%), negatively restricted (≤ 15%), or polytypic (16-84%) in T cells, using CD3 and/or TdT staining as the denominator

SUBMITTER: Chaudhary S 

PROVIDER: S-EPMC12701855 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature