In vitro production significantly reduces metabolic differences among bovine embryos.

ABSTRACT:

Introduction

Although the in vitro production of bovine embryos now exceeds in vivo production, the quality of in vitro-produced embryos remains inferior. Metabolomic analysis of the spent culture medium used for embryos is considered a relevant source of markers for subsequent developmental capacity, genetic status and sex. However, little is known about the sources of variability in this metabolome and their respective significance.

Objective

We compared bovine embryo spent culture media from in vivo developed (IVD) and in vitro produced (IVP) embryos, and analyzed how they varied with embryo stage, grade and sex.

Methods

Embryos were produced in vitro under two different conditions: SOF medium supplemented with 1% fetal calf serum or IVF Bioscience media, or develo

SUBMITTER: Laloe D 

PROVIDER: S-EPMC12701867 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature