Sulfonylation sites for adenine and its nucleosides/nucleotides.

ABSTRACT: Sulfonylation is extensively used to label DNA and RNA, assess their interactions, and quantify components including nucleobases and nucleosides/nucleotides although the sulfonylation sites remain controversial. Here, we systematically investigated the sulfonylation of adenine (ade) and its nucleosides/nucleotides with 5-(dimethylamino)-naphthalene-1-sulfonyl chloride (DNS-Cl), 5-(diethylamino)-naphthalene-1-sulfonyl chloride (DEANS-Cl), and 5-((N,N-diethylleucyl)amino)-naphthalene-1-sulfonyl chloride (DELANS-Cl). Detailed spectral analysis with nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HRMS) showed similar sulfonylation behaviors among the reagents. For ade, its secondary amine in the imidazole ring (N⁹H) sulfonylated mor

SUBMITTER: Cheng X 

PROVIDER: S-EPMC12701998 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature