Generation of a conditional <i>Adamts6</i> mouse allele reveals roles in lung maturation in addition to cardiac and musculoskeletal development.

ABSTRACT: Prior analysis of mouse embryos homozygous for a point mutation (p.Ser149Arg) that abrogated secretion of the protease, ADAMTS6, showed that it is essential for cardiovascular and limb development. Because Adamts6 ^S149R/S149R mice do not survive past birth, it is currently not feasible to investigate ADAMTS6 in specific cellular lineages postnatally. Therefore, we generated a conditional allele using CRISPR-Cas9-mediated genome editing to insert unidirectional loxP sites flanking the first coding exon in Adamts6, resulting in a frameshift mutation after loxP recombination. Mice homozygous for the unrecombined floxed allele (Adamts6 ^fl/fl) are viable, fertile, and without overt phenotype. Adamts6 ^del/del embryos, generated upon co

SUBMITTER: Sirek JM 

PROVIDER: S-EPMC12702026 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature